LOCUS       40924_7081        6716 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS 35S attR-Rluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Subramanian C, Woo J, Cai X, Xu X, Servick S, Johnson CH, Nebenfuhr 
            A, von Arnim AG.
  TITLE     A suite of tools and application notes for in vivo protein 
            interaction assays using bioluminescence resonance energy transfer 
            (BRET)
  JOURNAL   Plant J. 48 (1), 138-152 (2006)
  PUBMED    16925598
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   von Arnim AG, Subramanian C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2005) BCMB, University of Tennessee Knoxville, 
            1406 Circle Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 6716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "48"; issue: "1"; pages: "138-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2005) BCMB, University of Tennessee Knoxville, 1406 Circle 
            Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      1460..1602
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     protein_bind    1608..1732
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1757..1787
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1841..2497
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2842..3144
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3188..3312)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3314..4246
                     /label=hRluc
                     /note="Renilla luciferase"
     regulatory      4259..4469
                     /label=35S cauliflower mosaic virus terminator
                     /note="35S cauliflower mosaic virus terminator"
                     /regulatory_class="terminator"
     promoter        complement(4531..4549)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4570..4586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4594..4610)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4618..4648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4663..4684)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4972..5560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5734..6591)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6592..6696)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca ggtcaacatg
      721 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      781 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      841 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      901 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      961 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1021 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     1081 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     1141 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     1201 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1261 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1321 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1381 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1441 tcatttcatt tggagaggac ctcgagaatt ctcaacacaa catatacaaa acaaacgaat
     1501 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     1561 aagcaatttt ctgaaaattt tcaccattta cgaacgatag gcctaggaca agtttgtaca
     1621 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1681 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     1741 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     1801 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1861 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1921 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1981 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2041 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2101 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2161 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2221 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2281 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2341 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2401 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     2461 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     2521 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     2581 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg
     2641 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     2701 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     2761 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     2821 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     2881 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     2941 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     3001 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     3061 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     3121 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     3181 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3241 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3301 gtacaaagtg gtcatggctt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     3361 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     3421 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     3481 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     3541 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     3601 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     3661 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     3721 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     3781 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     3841 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     3901 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     3961 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     4021 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     4081 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     4141 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     4201 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaaagat ctatctagag
     4261 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     4321 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     4381 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     4441 ttctaattcc taaaaccaaa atccagtgac ctgcagcccg ggggatccac tagagcggcc
     4501 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     4561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5401 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgc
//
